rabbit pmkk4  (Cell Signaling Technology Inc)

Journal: Cancer Cell

Article Title: Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity

doi: 10.1016/j.ccell.2021.06.017

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Article Snippet: Anti-human/mouse pMKK4 [S257] clone C36C11 , Cell Signaling Technology , 4514.

Techniques: Blocking Assay, Virus, Recombinant, Plasmid Preparation, Saline, Lysis, Staining, Reverse Transcription, In Vivo, Electroporation, Mass Cytometry, Conjugation Assay, Illumina Sequencing, Library Quantification, In Vitro, Quantitative RT-PCR, Software, Real-time Polymerase Chain Reaction, Cytometry, Microscopy, Imaging, Spectrophotometry

Journal: bioRxiv

Article Title: IRAK1-mediated coincidence detection of microbial signals licenses inflammasome activation

doi: 10.1101/2019.12.26.888776

Figure Lengend Snippet: (A-C, E-G, I) Kdo-2 Lipid A and P3C co-treatment (50 nM each) in iBMDMs. (D, H) Co-TLR (PAMP: Kdo-2 Lipid A and P3C, 50 nM each) or multi-PRR priming (heat-killed Yersinia pseudotuberculosis , MOI 100) followed by Nigericin (10 μ M) trigger for 2h. (A) Quantification of the Pearson’s correlation coefficient (PCC) for the correlation of pJNK antibody staining in IRAK1 clusters. (B) Quantification of the Mander’s colocalization coefficient (MCC) for the co-occurrence of pJNK antibody staining in IRAK1 clusters. (C) pJNK staining in Irak1 −/− iBMDMs. (D) IRAK1-pJNK PLA. (E) PCC and (F) MCC for pMKK7 antibody staining in IRAK1 clusters. (G) pMKK7 staining in Irak1 −/− iBMDMs. (H) IRAK1-pMKK7 PLA in BMDMs. (I) pMKK4 staining in iBMDMs mIRAK1mCherry. (Panels A, B, E, F) Each data point represents value from a randomly selected non-over lapping field of cells. Paired t test. Data are represented as mean ± SD. Automated imaging of at least 2 (Panels C, G, I) fields of cells. At least 20000 (Panels D, H) cells imaged. p = 0.1234 (ns), 0.0332(*); 0.0021 (**); 0.0002 (***); < 0.0001 (****). Data shown are representative of at least two independent experiments.

Article Snippet: Primary – IRAK1 Rb (CST 4504S), IRAK1 Rb (Proteintech 10478-2-AP), IRAK1 Ms (SCBT sc-5288), ASC Rb (SCBT sc-22514-R), ASC Rb (CST 67824S), ASC Ms (SCBT sc-514414), ASC Ms (EMD Millipore 04-147), pASC Rb (ECM Biosciences AP5631), NLRP3 Ms (AdipoGen AG-20B-0014-C100), NLRC4 Rb (EMD Millipore 06-1125), pJNK1/2 Rb (Invitrogen 700031), IRAK4 Rb (CST 4363S), IRAK3 Rb (Thermo Scientific PA5-19969), IRAK2 Rb (Abcam ab66017), IRAK2 Ms (SCBT sc-515885), MyD88 Rb (CST 4283S), MyD88 Gt (R&D Systems AF3109), Rb IgG Isotype (CST 3900S), Ms IgG Isotype (CST 5415S), TRAF6 Rb (Bioss bs-1184R), TRAF6 Rb (Abcam ab33915), pTBK1 Rb (CST 5483S), pJNK Rb (Invitrogen 700031), pMKK4 Rb (CST 4514P), pMKK7 Rb (CST 4171S), p44/42 Rb (CST 4695P), p65 Rb (Abcam ab16502), pATF2 Ms (SCBT sc-8398), pATF2 Rb (CST 5112S), Pellino-1 (CST 31474S), TICAM2 Ms (SCBT sc-376076), pERK5 Rb (CST 3371S), pTAK1 Rb (CST 4531S), pTAB2 Rb (CST 8155S), βTrCP Rb (CST 11984S), Proteasome 20S alpha 5 Rb (Novus Biologicals NBP1-86838).

Techniques: Staining, Imaging